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Old 09-28-2008, 07:44 PM #1
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Default PerkinElmer 377 DNA Sequencer

Here's a description of the PerkinElmer 96 Lane 377 DNA Sequencer disassembly and important component list. Daguin turned me onto this sequencer located in Ann Arbor, MI being sold on eBay. I purchased it for $100.00 and drove down and picked it up. The first thing to keep in mind; it's heavy! Bring a friend and a truck to haul it. This sequencer is completely modular and comes apart very quickly.

This is what it looks like:


You know there is something good inside when you see a warning like this:


Panel Doors
The right front door opens with a latch and the left door opens by removing two hex bolts. The JDS Argon Laser is located at the bottom of the unit and the related power supply is at the top left of the unit.


Power Supply
Here's a closeup of the argon laser power supply. It has a key switch set to the on position.


Exposing the Laser
Removing two cover screws allows the argon laser to be visualized.


Cooling Fan
The cooling fan sits behind the laser at the back, lower left of the chassis.


Remove the Power Supply

Unscrew two screws, disconnect all connectors to the power supply and the power supply will pull free.


Remove the Laser
Unscrew two more mounting screws and the laser is free. I removed the plastic cover over the face of the unit by removing four hex bolts located under the sample securing knobs. Remove three screws to separate the laser from the cooling tube.


Removing the Cooling System
I removed the rest of the sample plate by unscrewing several more hex bolts. It just made it easier to visualize the cooling fan. Four screws secure the fan to the chassis and it slips free.










So what all did I get for my $100.00? Let's look:

A nice JDS Argon Laser and power supply


The cooling system for the laser


A 18V NiCd battery pack


A circuit board that is not usable in and of itself but contains several nice heat sinks and DC-DC converters that can be desoldered.


A multiple output DC power supply. This system supplied power to the circuit board and a bunch of smaller components. It's also modular allowing each of the sub-power supplies to be removed.


A 240VAC power distribution system.


A complete closed circuit cooling system including pump, cooling fan, and radiator with valves to allow for external cooling water.


A periscope beam turning system with dual solenoid shutters and a beam splitter.


Finally, a diffraction measuring system which probably has some nice optics inside. There is also a huge heatsink mounted to this device. I'll dig into this component later.



These sequencers are really worth getting if you have a chance. They are loaded with nice components and, of course, the beautiful argon goodness. Once I get the laser mounted and running I'll add some beam shots and power information.


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Old 09-28-2008, 07:54 PM #2
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Default Re: PerkinElmer 377 DNA Sequencer

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Old 09-28-2008, 08:07 PM #3
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Default Re: PerkinElmer 377 DNA Sequencer

Wow, thats an awesome find!
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Old 09-28-2008, 08:17 PM #4
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Default Re: PerkinElmer 377 DNA Sequencer

Quote:
Originally Posted by daguin
Awww daves all chuffed :

Nice one Frothy Chimp, having fun with that argon ?
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Old 09-28-2008, 08:59 PM #5
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Default Re: PerkinElmer 377 DNA Sequencer

is that a 2214-40MLA???

Very nice!
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Old 09-28-2008, 11:53 PM #6
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Default Re: PerkinElmer 377 DNA Sequencer

Also keep an eye out in university surplus stores (if your local campus has them), those places are goldmines for equipment that you can't get anywhere else! I just hope I'm not encouraging more people to visit MY local one!
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Old 09-30-2008, 10:52 PM #7
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Default Re: PerkinElmer 377 DNA Sequencer

There's one for sale on ebay now.-&gt;
http://cgi.ebay.com/BECKMAN-GX100-Ge...3286.m20.l1116

It also is an electrophoresis based sequencer. So there's a laser in there somewhere. If I was in Texas I'd go get it. (They want $450 for shipping!)

cheers,
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Old 09-30-2008, 11:03 PM #8
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Default Re: PerkinElmer 377 DNA Sequencer

Quote:
Originally Posted by kernelpanic
There's one for sale on ebay now.-&gt;
http://cgi.ebay.com/BECKMAN-GX100-Ge...3286.m20.l1116

It also is an electrophoresis based sequencer. So there's a laser in there somewhere. If I was in Texas I'd go get it. (They want $450 for shipping!)

cheers,

STOP!!!!!!



That is the GX100 *The GX is the electrophoresis unit ONLY. *There is NO LASER in it. *The DNA plates are &quot;grown&quot; in this unit, but the plates must be moved to another unit (the FL100) to fluoresce the DNA. *Now if you find an FL100, it should have a 40mW &quot;single line&quot; in it.

Peace,
dave
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Old 09-30-2008, 11:25 PM #9
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Default Re: PerkinElmer 377 DNA Sequencer

Daguin,

Thanks for the heads up!

cheers,
kernelpanic
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Old 10-01-2008, 12:39 AM #10
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Default Re: PerkinElmer 377 DNA Sequencer

Quote:
Originally Posted by kernelpanic
Daguin,

Thanks for the heads up!

cheers,
kernelpanic

You're welcome

Peace,
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