Laser pointer discussion. Read/write reviews of laser pointers and laser pointer companies. Learn about all types of laser pointers and lasers





Welcome to Laser Pointer Forums! If you are looking for a laser pointer or want to compare different laser pointer companies, you may want to check out the LPF Laser Pointer Company Database. The link will open in a new window for your convenience.








Green Laser Pointers by Category Blue Laser Pointers by Category Red Laser Pointers by Category
Yellow Laser Pointers by Category Violet Laser Pointers by Category Orange Laser Pointers by Category
Top Laser Pointers by Power - 1 Watt+ Top Laser Pointers by Power - 500mW+ Top Laser Pointers by Power - 250mW+
Laser Pointer Database High Power Laser Pointers Laser Pointer Diodes
Laser Technology Laser Pointer Parts Lasers by Wavelength
Top 10 Laser Pointer Companies Laser Pointer Company Database Visible Beam Laser Pointers


One laser store meets all your needs

























Go Back   Laser Pointer Forums - Discuss Laser Pointers > Lasers > Blue, Yellow, & Other Colored Lasers



LPF List of Laser Pointer Companies (link opens new window)



Reply
 
LinkBack Thread Tools Display Modes
Old 04-08-2012, 06:18 AM #1
Junior Member
 
Join Date: Apr 2012
Location: Seattle
Posts: 2
Rep Power: 0
DPSci is on a distinguished road
DPSci DPSci is offline
Junior Member
 
Join Date: Apr 2012
Location: Seattle
Posts: 2
Rep Power: 0
DPSci is on a distinguished road
Question Making a FRAP microscope on the cheap?

Here's the story, I'd like to be able to do FRAP experiments in my lab(biochemist here). I basically want to illuminate a single spot on my sample for around 50ms, which will photobleach it. I don't need to make weird shapes or even use multiple wavelengths (I'll need 488nm for my fluorophore).

Unfortunately Nikon FRAP microscopes are $200,000. While they are nice, I don't have that ind of cash .

Is there an easy way for me to optically focus a cheaper ~$6K Tem00 laser to a 30-50um diameter spot?

There's a paper/video in the literature where they use a dye cell laser. Those cost around $40,000. Is there any reason I can't just find a lens to focus the Tem00 laser I linked to earlier?

If this setup works, it will come in at around $40K total and I plan to publish the design in an open access journal(probably PLoSone), or if it gets rejected on a website. Thanks for your help, I really don't know much about lasers, but hopefully where there's a will there's a way.

I have 2 pairs of OD 6 safety glasses already ordered btw



Last edited by DPSci; 04-08-2012 at 06:25 AM. Reason: added video
DPSci is offline   Reply With Quote







LPF List of Laser Pointer Companies (link opens new window)







Old 04-08-2012, 03:47 PM #2
Cyparagon's Avatar
Class 4 Laser
 
Join Date: Sep 2007
Location: Wisconsin
Posts: 9,142
Rep Power: 9321
Cyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond repute
Cyparagon Cyparagon is online now
Class 4 Laser
Cyparagon's Avatar
 
Join Date: Sep 2007
Location: Wisconsin
Posts: 9,142
Rep Power: 9321
Cyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond repute
Default Re: Making a FRAP microscope on the cheap?

Did you already buy it? If all you need is a focused 488nm beam, a used argon laser with a short FL lens will work. <$500. I don't think it would get you a publication though.
__________________
A problem well stated is a problem half solved.
Cyparagon is online now   Reply With Quote
Old 04-08-2012, 05:20 PM #3
Junior Member
 
Join Date: Apr 2012
Location: Seattle
Posts: 2
Rep Power: 0
DPSci is on a distinguished road
DPSci DPSci is offline
Junior Member
 
Join Date: Apr 2012
Location: Seattle
Posts: 2
Rep Power: 0
DPSci is on a distinguished road
Default Re: Making a FRAP microscope on the cheap?

No, I haven't bought anything related to the laser yet. Do you have any opinions on brands to avoid while I look on ebay?

Do you think this lens would be ok for me?
DPSci is offline   Reply With Quote
Old 04-08-2012, 08:35 PM #4
Cyparagon's Avatar
Class 4 Laser
 
Join Date: Sep 2007
Location: Wisconsin
Posts: 9,142
Rep Power: 9321
Cyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond repute
Cyparagon Cyparagon is online now
Class 4 Laser
Cyparagon's Avatar
 
Join Date: Sep 2007
Location: Wisconsin
Posts: 9,142
Rep Power: 9321
Cyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond reputeCyparagon has a reputation beyond repute
Default Re: Making a FRAP microscope on the cheap?

Shorter FL will result in a smaller spot. They can be found much cheaper. Here for example.
__________________
A problem well stated is a problem half solved.
Cyparagon is online now   Reply With Quote
Reply

Tags
focusing, frap, microscopy





Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On











Loading








Green Laser Pointers by Category Blue Laser Pointers by Category Red Laser Pointers by Category
Yellow Laser Pointers by Category Violet Laser Pointers by Category Orange Laser Pointers by Category
Top Laser Pointers by Power - 1 Watt+ Top Laser Pointers by Power - 500mW+ Top Laser Pointers by Power - 250mW+
Laser Pointer Database High Power Laser Pointers Laser Pointer Diodes






Privacy Policy | Advertising Disclaimer | Terms of Use


 


All times are GMT. The time now is 01:54 AM.


Powered by: vBulletin
Copyright ©2000 - 2017, Jelsoft Enterprises Ltd.
Search Engine Friendly URLs by vBSEO
Privacy Policy | Advertising Disclaimer | Terms of Use
Copyright (C) 2017 Laser Pointer Forums, LLC