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Old 01-17-2010, 10:28 PM #1
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Default Looking for Fluorescein Sodium

^^


Quote:
Chemical and physical properties
Fluorescein under UV illumination

The fluorescence of this molecule is very high, and excitation occurs at 494 nm and emission at 521.

Fluorescein has a pKa of 6.4 and its ionization equilibrium leads to pH-dependent absorption and emission over the range of 5 to 9. Also, the fluorescence lifetimes of the protonated and deprotonated forms of fluorescein are approximately 3 and 4 ns, which allows for pH determination from non-intensity based measurements. The lifetimes can be recovered using time-correlated single photon counting or phase-modulation fluorimetry.
http://en.wikipedia.org/wiki/Fluorescein




It goes for around 1$ a gram, but maybe we can get alot of these in a GB's for much cheaper.
I am looking for about 20-30 grams.

-Ranged


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Last edited by rangedunits; 01-17-2010 at 10:40 PM.
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Old 01-17-2010, 11:55 PM #2
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Default Re: Looking for Fluorescein Sodium

I have a bottle of concentrate in the laundry room rangedunits, bring me a little bottle and I'll pour ya a few dozen mls no charge pal! -Glenn
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Bluray 140 mW, DJR & New FAV! 8X >500 mW Cree! Made by IgorT Flaminpyro BR &
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Rigel SOLD! 3-4 mW I Love it PGL III 593.5nm doing >20 mW! SOLD!
Several Green lasers, EVO PRO Peak ~160 mW & Spyder I Peak 295 mW are my new green favorites!
LG Aquarius -10 & Viasho 473 nm peak ~29 mW WL S3 >60 mW peak 112mW SOLD!
Awesome rhd 451nm 2.1 W SOLD! & 445 >2W by DTR! , Yobresal 1.2W Cree & pen 445 w/ star-cap doing 750 mW!! 500mW 445nm Dorcy Jr. Thanks to Flaminpyro!
Awesome Osram 450nm pocket mini from DTR does about 750mW!

Big T/Y! to builders: IgorT, DTR, john lawson, jayrob, IgorT, whatsupadrian, daguin, and all!
Spiro toy mod w/ side aperture for any pointer Spiro toy in Violet Spiro by yobresal too!
Laser sleds/ diodes sold so far 1180+ A few LPC-826 diodes F.S. $12 ea.



Last edited by scopeguy20; 01-18-2010 at 12:02 AM.
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Old 01-18-2010, 12:00 AM #3
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Default Re: Looking for Fluorescein Sodium

You have it in liquid form?



Keep the diode from the lpf raffle, i have no use for it.
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Old 01-18-2010, 12:04 AM #4
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Default Re: Looking for Fluorescein Sodium

I added a pic!

It's so concentrated a BR laser only penetrates about 1 inch. -GH
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Bluray 140 mW, DJR & New FAV! 8X >500 mW Cree! Made by IgorT Flaminpyro BR &
Red Burner on board, need more ~650 nm 140 mW
8 red Hene's 632.8 nm 2-3mW green HeNe & T/Y to rhd 635nm ~400mW
Rigel SOLD! 3-4 mW I Love it PGL III 593.5nm doing >20 mW! SOLD!
Several Green lasers, EVO PRO Peak ~160 mW & Spyder I Peak 295 mW are my new green favorites!
LG Aquarius -10 & Viasho 473 nm peak ~29 mW WL S3 >60 mW peak 112mW SOLD!
Awesome rhd 451nm 2.1 W SOLD! & 445 >2W by DTR! , Yobresal 1.2W Cree & pen 445 w/ star-cap doing 750 mW!! 500mW 445nm Dorcy Jr. Thanks to Flaminpyro!
Awesome Osram 450nm pocket mini from DTR does about 750mW!

Big T/Y! to builders: IgorT, DTR, john lawson, jayrob, IgorT, whatsupadrian, daguin, and all!
Spiro toy mod w/ side aperture for any pointer Spiro toy in Violet Spiro by yobresal too!
Laser sleds/ diodes sold so far 1180+ A few LPC-826 diodes F.S. $12 ea.



Last edited by scopeguy20; 01-18-2010 at 12:07 AM.
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Old 01-18-2010, 12:07 AM #5
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Default Re: Looking for Fluorescein Sodium

Doesn't it should look orange if its concentrate?
mine was orange when i pourred too much powder
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Old 01-18-2010, 12:13 AM #6
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Default Re: Looking for Fluorescein Sodium

Depends on several things, here it is shown with a 3/4 W 365nm LED light. -GH
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Bluray 140 mW, DJR & New FAV! 8X >500 mW Cree! Made by IgorT Flaminpyro BR &
Red Burner on board, need more ~650 nm 140 mW
8 red Hene's 632.8 nm 2-3mW green HeNe & T/Y to rhd 635nm ~400mW
Rigel SOLD! 3-4 mW I Love it PGL III 593.5nm doing >20 mW! SOLD!
Several Green lasers, EVO PRO Peak ~160 mW & Spyder I Peak 295 mW are my new green favorites!
LG Aquarius -10 & Viasho 473 nm peak ~29 mW WL S3 >60 mW peak 112mW SOLD!
Awesome rhd 451nm 2.1 W SOLD! & 445 >2W by DTR! , Yobresal 1.2W Cree & pen 445 w/ star-cap doing 750 mW!! 500mW 445nm Dorcy Jr. Thanks to Flaminpyro!
Awesome Osram 450nm pocket mini from DTR does about 750mW!

Big T/Y! to builders: IgorT, DTR, john lawson, jayrob, IgorT, whatsupadrian, daguin, and all!
Spiro toy mod w/ side aperture for any pointer Spiro toy in Violet Spiro by yobresal too!
Laser sleds/ diodes sold so far 1180+ A few LPC-826 diodes F.S. $12 ea.



Last edited by scopeguy20; 01-18-2010 at 12:34 AM.
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Old 01-18-2010, 12:22 AM #7
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Default Re: Looking for Fluorescein Sodium

Alright, remember the toxic barell i sent you a while ago?

If its still laying around, you can use it, but the seal sucks so i don't know...tape around it if possible.
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Old 01-18-2010, 12:47 AM #8
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Default Re: Looking for Fluorescein Sodium

Quote:
Originally Posted by rangedunits View Post
^^



Fluorescein - Wikipedia, the free encyclopedia




It goes for around 1$ a gram, but maybe we can get alot of these in a GB's for much cheaper.
I am looking for about 20-30 grams.

-Ranged
Sodium Fluorescin can be found in quite pure form inside yellow-green highlighters. Get a big highlighter let the dye from the highlighter concentrate and add Alcohol, problem sloved.

I have used this exact method for making a UV pumped Dye laser. It worked well! I used Ethyl Alcohol as the solvent.
I think your solution needs to be thinner. --- this is why I suggest using Alcohol!
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